Fig. 4: Hepatic Mc3r reactivation reversed fat accumulation and fatty acid metabolism in Mc3r insufficient mice.
From: Melanocortin 3 receptor regulates hepatic autophagy and systemic adiposity
a H&E staining (b) Oil Red O staining for liver tissue from Mc3r+/+, Mc3rTB/TB and Mc3rHep/Hep. c Fold change of liver TG levels from Mc3r+/+ (n = 8), Mc3rTB/TB (n = 8) and Mc3rHep/Hep (n = 7) independent observations. d Liver weight (g) from Mc3r+/+ (n = 36), Mc3rTB/TB (n = 11) and Mc3rHep/Hep (n = 24) independent observations (e) eWAT weight (g) from Mc3r+/+ (n = 33), Mc3rTB/TB (n = 15) and Mc3rHep/Hep (n = 24) independent observations (f) Serum TG measured from Mc3r+/+ (n = 6), Mc3rTB/TB (n = 7) and Mc3rHep/Hep (n = 5) independent observations. g Serum non-esterified fatty acids (NEFA) from Mc3r+/+ (n = 6), Mc3rTB/TB (n = 9) and Mc3rHep/Hep (n = 9) independent observations. h Serum glycerol from Mc3r+/+ (n = 6), Mc3rTB/TB (n = 9) and Mc3rHep/Hep (n = 9) independent observations. i Serum TC from Mc3r+/+ (n = 6), Mc3rTB/TB (n = 9) and Mc3rHep/Hep (n = 6) independent observations. j qPCR mRNA measurements for fatty acid metabolism from Mc3r+/+ (n = 8 fed) and (n = 7 fasted) independent observations, Mc3rTB/TB (n = 8), and Mc3rHep/Hep (n = 6) independent observations from both fed-fasted conditions. Data are represented as mean ± SEM. Groups were compared by one-way ANOVA followed by Tukey’s HSD test (c–f), one-way ANOVA followed by Fisher’s LSD test (g–i), and two-way ANOVA followed by Tukey’s HSD test (j). * p < 0.05; ** p < 0.01; *** p < 0.001, Scale bar, 50 µm (a, b). Source data are provided as a Source Data file.
