Fig. 5: Assessment of energy intake and energy expenditure at ambient and thermoneutral temperatures by mice indirect calorimetry.
From: Melanocortin 3 receptor regulates hepatic autophagy and systemic adiposity
a Cumulative energy intake (kcal) monitored for light and dark phase at ambient 22 °C and thermoneutral temperature 30 °C. b Daily energy intake (kcal) for light, dark, and total (24 h) at 22 °C from Mc3r+/+, Mc3rTB/TB and Mc3rHep/Hep (n = 20) independent observations. c Daily energy intake (kcal) for light, dark, and total (24 h) at 30 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. d Total energy expenditure (kcal/mouse/h) for light and dark phase e TEE (kcal/mouse/h) for light, dark, and total (24 h) at 22 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. f TEE (kcal/mouse/h) for light, dark, and total (24 h) at 30 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. g Oxygen consumption (VO2) (ml/mouse/h) for light and dark phase h VO2 (ml/mouse/h) for light, dark, and total (24 h) at 22 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. i VO2 (ml/mouse/h) for light, dark, and total (24 h) at 30 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. j Respiratory exchange ratio (RER) (VCO2/VO2) for light and dark phase (k) RER (VCO2/VO2) for light, dark, and total (24 h) at 22 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. l RER (VCO2/VO2) for light, dark, and total (24 h) at 30 °C from Mc3r+/+, Mc3rTB/TB, and Mc3rHep/Hep (n = 20) independent observations. In (a, d, g, and j), data from the first day of adaptation are not shown. Chow-fed, n = 20 mice/group (10 males and 10 females) mice 3–4 months of age. Data are represented as mean ± SEM. Groups were compared using Friedman’s test by Dunn’s paired analysis (b, c, e, f, h, i, k, l) and post hoc test for adjusted estimated marginal means in Supplementary Table 1. * p < 0.05; ** p < 0.01; *** p < 0.001. Source data are provided as a Source Data file.
