Fig. 3: Microtubule dynamics are unaffected in DCX-KO neurons.

A (Top) Immunostaining representative of 3 experiments showing CTRL0 and DCXKO1 neurons at DIV3 with an α-Tubulin (DM1A) specific antibody, and (Bottom) mean intensity profile found in the distal tip of neurons from both genotypes, SEM are shown in lighter gray (CTRL0) and lighter cyan (DCXKO1). Images included in the analysis are from n = 135 CTRL0 neurites and n = 134 DCXKO1 neurites from 3 experiments. B Schematics of the experimental procedure and analysis method for measuring microtubule dynamics. NPCs are electroporated with the plus-tip marker EB3-mCherry, and neurons are imaged at DIV3. Each comet is assigned to a domain (Shaft or growth cone (GC)), and its characteristics are measured: length, lifetime, growth rate, and direction. The EB3 snapshot and kymograph image are representative of 4 similar experiments, (C) Distribution of growth rates for plus end out comets in shaft or growth cone of CTRL0 and DCXKO1 neurons. D Distribution of lifetimes for plus end out comets in shaft or growth cone of CTRL0 and DCXKO1 neurons. E Distribution of the proportion (%) of minus end out (retrograde) comets in the shaft and growth cone of CTRL0 and DCXKO1 neurons. For clarity reasons, datapoints at 0 are not shown, but are included in the analysis. For (C–E), n = 63 CTRL0 and n = 63 DCXKO1 cells from 4 experiments. Statistical analysis was performed using the Mann-Whitney non parametric U test: ns nonsignificant, *p < 0.05. p-values are detailed in Supplementary Table 1.