Fig. 4: Septin family gene expression and PRDM10-dependent regulation in early development.

a Heat maps showing mRNA transcription of Septin family genes during oogenesis²⁰ (RPKM), OET and preimplantation development¹⁹ (FPKM). b Heat map showing RPFs (ribosome protected fragments) of Septins in oocytes and preimplantation embryos¹⁹ (FPKM). c Heat map showing protein intensities of Septin family members in oocytes and preimplantation embryos by LC-MS/MS⁵⁷. Septin members are clustered in functional homology groups. d Predicted Septin hexamer complex based on transcription and proteomic data in Ctr (left) and Prdm10 MatKO oocytes (right). e Expression heatmap from RNA-seq data from mouse Ctr/MatKO oocytes, Ctr/ZygKO 8-cell embryos and Ctr/iKO mESCs, respectively. Septin members are clustered in groups based on structural and functional similarity (column 1). PRDM10 promoter-binding as defined by ChIP-seq in mESCs is indicated (column 2). Only Sept11 is bound by PRDM10 and regulated by PRDM10 (red box/asterisk) in oocytes and mESCs. f Schematic illustration of oocyte activation and staining protocol to detect maternal Septin complex at polar body abscission site. g Immunofluorescence detection of core Septin members 2, 7 and 11 at the polar body abscission site in parthenogenically activated Ctr/MatKO MII oocytes, respectively (DNA blue; SEPTIN2/7/11 green; TUBULIN red). In Ctr parthenotes, SEPTIN2 (n = 22), 7 (n = 13) and 11 (n = 12) were all localized at the cytokinetic ring (white arrowheads) where abscission of the polar body will occur. In MatKO parthenotes only SEPTIN2 (n = 17) was detected at the cytokinetic ring (white arrowheads); SEPTIN11 (n = 12) and 7 (n = 11) were absent (scale bar 50 μm).