Fig. 5: Partial rescue of SEPTIN7 recruitment and 2-cell block by Sept11 cRNA injection.

a Schematic of Sept11-eGFP cRNA injection rescue strategy for SEPTIN7 recruitment: GV oocytes are microinjected, matured to MII oocytes, parthenogenically activated and fixed and stained upon polar body extrusion. b Sept11-eGFP Ctr and MatKO injected and mock injected parthenotes were stained for eGFP (green/arrowhead), SEPTIN7 (red/arrowhead) and DNA (blue), respectively (scale bar 50 μm). Rightmost column shows magnification of the polar body abscission site (scale bar 25 μm). c Schematic diagram for 2-cell block rescue strategy: Zygotes were injected with Sept11-eGFP-cRNA and cultured beyond the 2-cell stage, up to blastocyst formation. d Quantification of in vitro development post-injection: 80% of wildtype embryos developed beyond the 2-cell stage (n = 52/66). Full 2-cell block was observed in mock-injected Prdm10 MatKO embryos (n = 0/12, rescued). Sept11-eGFP-cRNA injection enabled 22% of MatKO embryos to overcome the 2-cell block (n = 11/51, rescued). e Percentual distribution of developmental stage arrests of rescued MatKO embryos overcoming the 2-cell block. f Brightfield images of representative rescue embryos arrested at the 3-cell, 8-cell (2x) and blastocyst stage, respectively (scale bar 50 μm).