Fig. 4: Activating PKM2 reverses the protective effect of GMRSP in both in vitro and in vivo.
From: GMRSP encoded by lncRNA H19 regulates metabolic reprogramming and alleviates aortic dissection
Seahorse glycolysis stress test (n = 8 biologically independent samples) (A), representative images of TMRE staining, Scale bar: 50 μm (B), F-actin and MitoTracker staining, Scale bar: 50 μm (C) were performed in human primary VSMCs transfected with the indicated constructs for 36 h, followed by TEPP-46 (20 µmol/L) and PDGF-BB (20 ng/mL) treatment for 24 h. D SM22α+GMRSPOE-flox mice were induced with tamoxifen for 5 days at two weeks. One week after the last tamoxifen treatment, mice were treated with 0.25% BAPN monofumarate and TEPP-46 (10 mg/kg) for four weeks (n = 10 biological replicates per group). E Representative images of the mouse aorta are shown. F Maximum aortic diameter measurement (n = 10 biological replicates per group). G Survival rate estimation using the Kaplan-Meier method and comparison using the Breslow test (n = 10 biological replicates per group). H Representative macroscopic images of aorta sections were stained with H&E and EVG, Scale bar: 200 μm and 50 μm. The wall thickness (I) and elastin integrity (J) of each groups were analyzed(n = 10 biological replicates per group). Data are presented as the mean ± SD. The p values were calculated by a two-tailed unpaired Student’s t-test in (A, F, I, J). The p values were calculated by Kaplan–Meier analysis and two-sided Breslow test (G). The data presented in (A, B, C, H) are representative of three independent experiments. Source data are provided as a Source Data file. BAPN, β-aminopropionitrile.
