Fig. 1: Whole body mouse fluorescence imaging reveals unique peripheral biodistribution patterns of ATVTfR, ATVCD98hc, and control IgG. | Nature Communications

Fig. 1: Whole body mouse fluorescence imaging reveals unique peripheral biodistribution patterns of ATVTfR, ATVCD98hc, and control IgG.

From: Fc-engineered large molecules targeting blood-brain barrier transferrin receptor and CD98hc have distinct central nervous system and peripheral biodistribution

Fig. 1

a Schematic of the molecules used with non-targeting control Fabs. The orange patch in ATVTfR Fc region binds to TfR and blue patch in ATVCD98hc binds to CD98hc. b Schematic of the experimental paradigm. TfRmu/hu KI, CD98hcmu/hu KI, or WT mice were dosed with 30 mg/kg AF647-conjugated control IgG and ATVTfR for 1 day and ATVCD98hc for 5 days. c Ventral and dorsal view 3D immunofluorescence images AF647-conjugated control IgG, ATVTfR, or ATVCD98hc in the whole mouse. Representative immunostaining from n = 3/group. Quantification of mean fluorescence intensity of AF647-ATVTfR (d) or AF647-ATVCD98hc (e) normalized to AF647-conjugated control IgG. n = 3/group, mean ± sem, two-tailed t-test with Benjamini–Hochberg false discovery rate, vertebrae adjusted p value 0.045, brain cerebrum adjusted p value 0.045, brain hippocampus adjusted p value 0.045, mean fluorescence intensity of ATV compared to control IgG. 3D reconstructed images of select peripheral organs with high ATVTfR (f) or ATVCD98hc (g) uptake. VNO vomeronasal organ, intra. l.g. intraorbital lacrimal gland, extra. l.g. extraorbital lacrimal gland. Representative images from n = 3/group.

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