Fig. 4: ATVTfR and ATVCD98hc localize to BBB vascular and CNS parenchymal cells while control IgG localizes to blood-CSF and perivascular barrier cells. | Nature Communications

Fig. 4: ATVTfR and ATVCD98hc localize to BBB vascular and CNS parenchymal cells while control IgG localizes to blood-CSF and perivascular barrier cells.

From: Fc-engineered large molecules targeting blood-brain barrier transferrin receptor and CD98hc have distinct central nervous system and peripheral biodistribution

Fig. 4

a Schematic of the experimental design: single cells obtained from dissociated brains of WT, TfR mu/hu KI, and CD98hcmu/hu KI mice dosed with 45 mg/kg AF647-conjugated antibodies were sorted based on AF647 signal and then loaded onto the 10x Genomics platform for scRNA sequencing. b Representative flow cytometry plots showing the percentage of AF647-positive cells and their percent distribution across the negative, low, mid, and high AF647 intensity bins. c UMAP of cell clusters captured from the dissociated brains of mice from all three treatment groups and across all four bins. d Bar graphs represent mean percent of total huIgG distribution across indicated cell types captured per treatment group across fluorescence intensity bins. Points represent individual mice. Numbers displayed represent the total number of cells captured for the respective cell type and fluorescent intensity bin. Control IgG (n = 2/group); ATVTfR and ATVCD98hc (n = 4/group).

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