Fig. 6: PGLYRP1 regulates immune responses in TNBS-induced colitis.

a Weight development of wild type and Pglyrp1-/- mice during TNBS-induced colitis (n = 4 per group). Immunofluorescence staining of PGLYRP1 and macrophages (F4/80) in colon sections of wild type and Pglyrp1-/- mice before (b), after TNBS induced colitis (c, d) TNBS colitis induction after 3 days of i.p. pretreatment with 100 μg of GMTriP-K in 50 μL PBS at day 9. e Quantitation of F4/80⁺ macrophages and percentage of PGLYRP1 expressing F4/80⁺ macrophages during TNBS colitis. Macrophages were counted in 4 independent regions per section and quantitated using Imaris 10.1.1 image analysis software. f Staining for PGLYRP1 and SEC61β in the colon of wild type mice with TNBS colitis. g qPCR analysis of gene expression in mesenteric lymph nodes of wild type and Pglyrp1-/- mice on day 9 of the TNBS and GMTPriP-K treatment protocol (n = 3–5). Scale bars represent 20 μm in (b, c, d) and 2 μm in (g). Data are presented as mean ± SEM with indicated P values analyzed by one-way ANOVA. All experiments were repeated twice and yielded consistent results.