Fig. 7: The PGLYRP1 receptor system is regulated in ulcerative colitis.
From: PGLYRP1-mediated intracellular peptidoglycan detection promotes intestinal mucosal protection
a Immunofluorescence staining of PGLYRP1 and macrophages (CD68) in tissue sections of healthy human colon and active ulcerative colitis (arrow indicates immune cells that crossed the epithelial barrier, cr, Crypt.). b Quantitation of CD68+ macrophages and percentage of PGLYRP1 expressing CD68+ macrophages in the normal colon and ulcerative colitis lamina propria. Macrophages were counted in 4 independent regions per sample and quantitated using Imaris 10.1.1 image analysis software. c Hierarchal cluster analysis of genes that were significantly regulated in active, inactive ulcerative colitis and healthy control colons. d Volcano plot analysis of gene expression comparing gene expression in healthy control and active ulcerative colitis. e qPCR analysis of expression of indicated genes in healthy control (n = 6), inactive (n = 8), and active ulcerative colitis (n = 6) samples. Scale bars represent 25 μm. Data are presented as mean ± SEM with indicated P values analyzed by one-way ANOVA. Student’s test was used to compare control and UC samples. All experiments were repeated at least twice and yielded consistent results.
