Fig. 3: Helicobacter pylori infections.

a Timeline brightfield images (gray, extended field of depth) and confocal images of bacteria (red, maximum intensity projection). Bacteria were introduced apically in either neutral or acidic medium at 7-day-old samples. Apical medium was exchanged every 24 h thereafter. Scale bars, 100 µm. b Confocal images (maximum intensity projection) of fixed and stained samples 6 days p.i. Overview image (left) and high-magnification image with split channel images (right). Scale bars, 100 µm (overview), 10 µm (zoom). Representative images from three independent experiments. c Assessment of colony forming units (CFUs) per sample 2 days and 6 days p.i. Each dot represents one of four independent samples. Indicated p-values result from a two-way ANOVA. Source data are provided as a Source Data file.