Fig. 5: Disruption of KGDH sensitizes cells to lipid peroxidation. | Nature Communications

Fig. 5: Disruption of KGDH sensitizes cells to lipid peroxidation.

From: Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson’s disease models

Fig. 5

A–D Immunoblots and quantifications of DLST, OGDH and DLD protein levels of non-targeting control (NT ctrl) and two independent lines of DLST knockdown (KD #1 and KD #2) HEK293T cells. N = 8 biological replicates. All data shown are mean ± SEM. ns, not significant. One-way ANOVA with Dunnett’s test. E–J The levels of unlabeled (M + 0) and 13C labeled (M + N) pyruvate, citrate/iso citrate, α-KG, succinate, fumarate and malate in non-targeting control and DLST knockdown HEK293T cells after 6 h of labeling with U-13C6 glucose. The levels of metabolites were normalized by the levels of 13C6 glucose for each sample. N = 5 biological replicates. All data shown are mean ± SEM. ns, not significant. Two-sided t test. K Histograms of the intensities of oxidized and reduced C11-BODIPY signals of control and DLST knockdown HEK293T cells under basal and RSL3-induced conditions. The ratio of oxidized to reduced C11-BODIPY fluorescence intensities of control and DLST knockdown cells under basal (L) and RSL3-treated (M) conditions. N = 4 biological replicates. All data shown are mean ± SEM. One-way ANOVA with Dunnett’s test. Source data are provided as a Source Data file.

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