Fig. 5: OMVs derived from engineered EcN can penetrate the intact gut barrier and enter the circulation.
From: Oral delivery of therapeutic proteins by engineered bacterial type zero secretion system
a Representative image of colonic sections after the injection of Cy5.5-labeled OMVs (abbreviated as “Cy5.5-OMV”) or PBS (abbreviated as control) into the colon lumen of healthy mice. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. b Representative images of different tissues (colon, liver and kidney) from healthy mice orally administered EcNΔnlpIΔthyA-SK or PBS. OMVs were detected using an anti-HA antibody (pink). Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. a, b Each experiment was repeated three times independently with similar results.
