Fig. 1: RIPK1 Kinase inhibition protects early-stage ileitis and colitis in newborn Tak1^IEC-KO mice, as well as ileitis and early-stage colitis in adult Tak1^tamIEC-KO mice.

a, b Representative images of ileum and colon sections from mice at P0 with indicated genotypes stained with H&E or immunostained for CC3 (a). Graphs showed the CC3⁺ signals in the ileum and colon of mice with the indicated genotypes (b). Scale bar, 100 μm. c Intestine length in mice with indicated genotypes at P0. d Kaplan–Meier survival curves of the indicated genotypes. e–h Representative images of the ileum (e) and colon (g) sections from mice at P4 with indicated genotypes stained with H&E or immunostained for CC3. Graphs showed the CC3⁺ signals in the ileum (f) and colon (h) of mice with indicated genotypes. Scale bar, 100 μm. i Intestine length in mice with indicated genotypes at P4. j The scheme of tamoxifen-induced TAK1 deletion. Created in BioRender. Yuan, J. (2025) https://BioRender.com/b69p319. k Kaplan–Meier survival curves of the mice with indicated genotypes. l Relative body weight curve of the mice with indicated genotypes. The body weight of each mouse was normalized to the weight of the day 0. m Intestine length of the mice at 3 dpi with indicated genotypes. n, o Representative images of ileum and colon sections from the mice at 3 dpi with indicated genotypes stained with H&E or immunostained for CC3 (n). Graphs showed the CC3⁺ signals in the ileum and colon of the mice with indicated genotypes (o). Scale bar, 150 μm for ileum; 100 μm for colon; p–s Representative images of the ileum (p) and colon (r) sections from the mice at 7 dpi with indicated genotypes stained with H&E or immunostained for CC3. Graphs showed the CC3⁺ signals in the ileum (q) and colon (s) of the mice with indicated genotypes. Anti-TNFα was treated in the indicated group from 1 day before tamoxifen injection every other day for a total of four times until sacrificed. Scale bar, 150 μm. Each dot represented one mouse. The results were shown as mean ± SEM. An unpaired two-tailed t-test was performed.