Fig. 5: Cooperation of TRADD and RIPK1 kinase in TLR signaling pathway.
From: Cooperation of TRADD- and RIPK1-dependent cell death pathways in maintaining intestinal homeostasis
a–f Tradd+/+ and Tradd−/− colon organoids were treated with 500 nM (5Z)-7-oxozeaeno and Pam3CSK4 (1 μg/ml) (a, b), LPS (1 μg/ml) (c, d) or Flagellin (500 ng/ml) (e, f) for 12 h with or without 50 μM Nec-1s. Cell death in organoids was determined by PI staining. Each dot represented one organoid. Scale bar, 50 μm. The results were shown as mean ± SEM. An unpaired two-tailed t-test was performed. g–l Primary BMDMs from the mice with indicated genotypes pretreated Pam3CSK4 (1 μg/ml) (g, h), LPS (100 ng/ml) (i, j) or Flagellin (100 ng/ml) (k, l) for 4 h and followed by 200 nM (5Z)-7-oxozeaeno. Cell death was measured by SytoxGreen, and the mean ± SEM from five biological replicates was shown (g, i, k). The levels of GSDMD, Caspase-1, Caspase-8, CC8, Caspase-3, CC3, RIPK1, and p-RIPK1(S166) were determined by immunoblotting (h, j, l).
