Fig. 6: RIPK1 kinase drives microbiota dysbiosis in the ileum of Tak1^tamIEC-KO mice.

a Chao1 alpha-diversity from mice of the indicated genotypes at 3 dpi. Each dot represented one sample. The results were shown as mean ± SEM. An unpaired two-tailed t-test was performed. b Principal coordinate analysis (PCoA) of 16S sequencing of ileum fecal at 3 dpi were analysed with Bray-Curtis distances (p = 0.001, PERMANOVA by Adonis) on the taxonomic profile (at the ASV level). c Average relative abundance of the top 30 abundant genera of ileum fecal at 3 dpi of mice with the indicated genotypes. d Cluster heat map of average relative microbiota abundance at genus level in the ileum of the mice with indicated genotypes at 3 dpi (cut-off: Tak1^fl/fl V.S. Tak1^tamIEC-KO p ≤ 0.05). * indicated Tak1^tamIEC-KO V.S. Tak1^tamIEC-KO Ripk1^D138N/D138N p ≤ 0.05. Z scores are shown. e Relative abundance of Enterococcus faecalis in ileum fecal of the mice with indicated genotypes at 3 dpi. Each dot represented one sample. The results were shown as mean ± SEM. An unpaired two-tailed t-test was performed. f Cluster heat map of AMP expression level in the ileum of the mice with indicated genotypes at 3 dpi (log₂|fold change | ≥1, p ≤ 0.05). Z scores are shown. Each column represented one mouse. g Ileal IECs from the mice with indicated genotypes at 3 dpi were isolated and immunoblotting for TAK1, RIPK1, Caspase-8, CC8, Caspase-3, CC3, p-ERK, ERK, p-P65, and P65.