Fig. 5: Effects of NP and VP24 mutations on RNA synthesis and NCLS formation.
From: Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24
a–c Transcription and replication activity evaluated using the minigenome assay with NP and VP24 mutants. The results are presented in box-and-whiskers plots. The plots detail the maxima, 75th percentile, median, 25th percentile, and minima, with outliers represented by dots. The activities are compared between a Wild-type VP24 and its mutants, with the activity of wild-type NP in the absence of VP24 normalized to 100%. b Wild-type NP and NP mutants, with the activity of wild-type NP normalized to 100%. (c) Wild-type NP and NP mutants, with the activity of each specific NP type in the absence of VP24 normalized to 100%. n = 3 for each technical replicate. All experiments were performed in three biological replicates (n = 9 in total). p values from ANOVA-Dunnett’s test are indicated as ***p <0.001, **p <0.01, *p < 0.05. d Ultrathin section electron microscopy of HEK293T cells co-expressing NP and VP24 mutants and VP35. Each lower panel shows a close-up view of the square area of the upper panel. Red arrows indicate the NCLS. Scale bars represent 100 nm. Source data are provided as a Source data file.
