Fig. 6: Intracellular transport of the NCLS composed of VP24 mutants.
From: Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24
a Maximum intensity projections of time-lapse images recorded for 3 min with 3 s intervals, showing the intracellular distribution of NCLS composed of wild-type VP24 and VP24 I35E, VP24 R59A, VP24 K148A, and VP24 N171A mutants. Scale bars represent 10 µm. The magnified image of the square area is displayed, with a scale of 2 µm. b Analysis of the number of NCLS showing long-range movement within the cell. Each point represents the number of moving NCLS per cell, with the average indicated by horizontal lines. Variants that are statistically significant compared to the wild type (wt) from one-sided Student’s t-test are marked with asterisks (*p < 0.05; ***p < 0.001, ****p < 0.0001). All experiments were performed in three biological replicates. c Analysis of the trajectory length of NCLS movement within cells. Data points represent the length of each NCLS movement, with the trend showing a significant reduction in movement distance for VP24 mutants compared to wild-type. Variants that are statistically significant compared to the wild type (wt) from one-sided Student’s t-test are marked with asterisks (*p < 0.05; ***p < 0.001, ****p < 0.0001). All experiments were performed in three biological replicates. Source data are provided as a Source data file.
