Fig. 3: EIF4E2 destabilizes CXCL10 mRNA to decrease CXCL10 protein level, reducing T cell tumor infiltration. | Nature Communications

Fig. 3: EIF4E2 destabilizes CXCL10 mRNA to decrease CXCL10 protein level, reducing T cell tumor infiltration.

From: PARP inhibitor radiosensitization enhances anti-PD-L1 immunotherapy through stabilizing chemokine mRNA in small cell lung cancer

Fig. 3

a Left: EIF4E2 and CXCL10 protein level in SBC5 with VEH or COM treatment at indicated time points from one of two independent experiments. Right: Quantification of EIF4E2 and CXCL10 protein level normalized by Actin on different days relative to the protein level at 0 h. b Relative CXCL10 mRNA level in EGFP KO or EIF4E2 KO for HEK293 and SBC5 cells (n = 4 biological replicates). c CXCL10 mRNA stability in EGFP KO control (Ctrl) or EIF4E2 KO cells for HEK293 treated with Dactinomycin (DACT) for 0, 2, 4 h (n = 3 biological replicates). d Western blot and quantification of CXCL10 protein levels in Ctrl or EIF4E2 KO for HEK293 or SBC5 cell-lines from one of two independent experiments. e Relative CXCL10 mRNA level in empty vector (Empty) or EIF4E2 overexpression for HEK293 and SBC5 cells (n = 3 biological replicates). f Left: overexpression of FLAG-EIF4E2 in HEK293 EIF4E2 KO cells. Right: CXCL10 mRNA level in HEK293 EIF4E2 KO cells expressing empty vector or FLAG-EIF4E2 after 4 h’ DACT treatment (n = 3 biological replicates). g Western blot and quantification of CXCL10 protein levels in Empty or V5-EIF4E2 overexpression for HEK293 or SBC5 cell-lines from one of two independent experiments. h Left: Schematic of SBC5-Empty or V5-EIF4E2 overexpression tumor engraftment in PBMC humanized NSG mice (Created in BioRender. Ran, X. (2025) https://BioRender.com/e93s595). Middle: Representative flow cytometry data for total T cells. Right: Cumulative data for total T cell (CD3+CD45+) in tumors on day 35 (n = 12). For (a), data are presented as individual dot plot and the correlation was analyzed by two-tailed Pearson correlation test. For (b), (c), (e) and (f), data are presented as mean ± SD and statistically analyzed by two-tailed, unpaired t test. For (h), data are presented as dot plot and statistically analyzed by the two-tailed, unpaired t test. (p: ****<0.0001 < *** < 0.001 < ** < 0.01 < * < 0.05). All exact p values are listed in the source data. Source data are provided as a Source Data file.

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