Fig. 5: Ex vivo infection of Vδ1 T cells from HIV− donors is CD4−dependent.
a Frequency of total circulating CD3 + Vδ1 (blue), Vδ2 (red), and αβ CD4 + T cells (green) in HIV− donors (n = 27). b Comparison of CD4 expression on Vδ1 (blue) and Vδ2 (red) T cells which did not correlate with (c) age or (d) biological sex of HIV− donors (male, blue; female, orange, n = 27). Comparison of co-receptors (e) CCR5 and (f) CXCR4 expressed on matched Vδ1 (blue), Vδ2 (red) and αβ CD4 (green) from HIV− donors (n = 15). g Representative pseudocolor plots and (h) frequency of infected Vδ1 (blue) and αβ CD4+ (green) T cells measured as positive for intracellular HIVp24 on day 7 of PBMCs from HIV− donors infected with HIVJR-CSF ex vivo (n = 9). i Frequency of CD4+ (blue) and CD4− (orange) Vδ1 T cells positive for HIVp24 on day 7 of infection of ex vivo PBMC infection (n = 9). j Correlation between the frequency of CD4 + Vδ1 T cells prior to infection and the frequency of HIVp24 + Vδ1 on day 7 of ex vivo PBMC infection (n = 9). k Comparison of the frequency of HIVp24 + Vδ1 T cells with or without α-CD4 blockade prior to infection (n = 9). Two-sided Wilcoxon matched pairs signed-rank test (b, e, f, h, I, k). Exact p value for (e) Vδ1 v. Vδ2 p = 0.00006 and Vδ2 v. αβ CD4 p = 0.00006. Two-sided Spearman’s r and p value shown for significant correlations with simple linear regression analyses showing the line of best fit and 95% confidence intervals (c, j). Mean ± S.E.M. are represented (a, b, d–f, h, i). Source data are provided as a Source Data file.
