Fig. 2: ACSS2 deficiency blunts CCF formation during senescence. | Nature Communications

Fig. 2: ACSS2 deficiency blunts CCF formation during senescence.

From: ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation

Fig. 2

a, b IMR90 cells were induced to senesce by oncogene RAS (a) or Etoposide (b). The proliferating cells and senescent cells expressing non-targeting shRNA, ACSS2, or ACLY-targeted shRNAs were infected with cGAS-FLAG encoding lentivirus. All groups of cells were subjected to γH2AX and cGAS-FLAG immunostaining. CCFs were indicated by arrows. Scale bar = 20 μm. c, d the CCF-positive cells were quantified among groups as described in (a, b). Randomly imaged fields with over 100 cells were analysed. Data represent the mean values of six different fields ± s.d. The P values were calculated using One-way ANOVA (Tukey’s multiple-comparison test). Representative of n = 3 independent experiments were shown (c, d). Source data are provided as a Source Data file.

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