Fig. 4: BOOST rapidly expands large biological specimens.
From: BOOST: a robust ten-fold expansion method on hour-scale
A Schematic of BOOST workflow for large biological specimens. B 3D visualization of an expanded whole airway organoid stained with NHS ester and SYTOX Green, EF = 10.1 ×. C 3D visualization (left) of an airway organoid stained with NHS ester, SYTOX Green, and antibody against β-tubulin IV, with additional views (middle and right) to reveal the ultrastructural details of cilia, EF = 10.0 ×. D Processing workflow of an intact lymph node to achieve a 9-fold expansion. Each square in the background represents a 5 mm × 5 mm area. E 2D image of the middle section of intact lymph node stained with ATTO 565 NHS ester, and (F) a magnified 3D view of the boxed region, EF = 9 ×. G 3D and 2D view of selected regions in the intact lymph node. Additional note: graphical icons in 4 A are created using Biorender. Scale bar (biological scale): 100 μm (B); 10 μm (C, left); 2 μm (C, middle); 0.2 μm (C, right); 100 μm (E); 10 μm (F); 2 μm (G).
