Fig. 2: Tie2-driven endothelial Myc-overexpression promotes neutrophil infiltration and exacerbates liver injury following BDL.
a Construction strategy of EC-specific Myc-overexpression mice. b qPCR analysis for Myc gene in 2-month-old mice (n = 5). c IF staining of CD31 (green) and Myc (red) in liver tissue samples from livers of (b). d quantitative analysis of Myc-positive ECs in liver tissue sections of (c) (n = 5). e Quantitative analysis of protein expression levels of Myc in ECs from MycLSL/+ and Tie2-MycLSL/+ mice by Fluorescence-Activated Cell Sorting (FACS) (n = 8). f Macroscopic view of livers from (b). g Serum enzyme levels in mice of (b) (n = 5). h qPCR analysis of indicated genes in livers from (f). Representative images (i) and quantitative analysis (j) of H&E and IHC for immune cell markers in livers from (f), with arrows indicating positive cells in respective panels (i). k Schematic diagram of BDL surgery and serum enzyme levels in BDL-treated mice (n = 5). l Macroscopic liver phenotype of mice in (k). Representative images (m) and quantitative analysis (n and o) of H&E and IHC for immune cell markers in livers from (l), with arrows indicating CD8A-positive cells and the red dotted box indicating the necrotic area (m). Data are presented as the mean ± SEM. P values are indicated as follows: * <0.05; ** <0.01; *** <0.001; **** <0.0001, ns = no significance. P values were obtained using the unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.
