Fig. 4: Increased susceptibility to BDL-induced liver injury in Lyve1-driven Myc-overexpression mice.

a Construction strategy for EC-specific Myc-overexpression mice. b qPCR analysis of Myc gene in the livers of 2-month-old mice (n = 5). c IF staining of CD31 (green) and Myc (red) in liver tissue samples from livers of (b). (d) quantitative analysis of Myc-positive ECs in liver tissue sections of (c) (n = 5). (e) Quantitative analysis of protein expression levels of Myc in ECs from Myc^LSL/+ and Lyve1-Myc^LSL/+ mice by FACS (n = 8). f Macroscopic view of livers from (b). g Serum enzyme levels in mice of (b) (n = 5). h, i qPCR analysis of indicated genes in livers of (f) (n = 5). j–l H&E, IHC staining of Ly6G, IF staining of CD31 (green) and P-selectin (red), and quantitative analysis (k–l) (n = 5, 6) in liver samples from the mice in (f), with arrows indicating positive cells in respective panels (j). m Schematic diagram of BDL surgery and serum enzyme levels in BDL mice (n = 5). n Macroscopic liver phenotype of the mice in (m). o H&E, IHC staining of Ly6G, IF staining of CD31 (green) and P-selectin (red), in liver samples from mice subjected to BDL. p–r Quantitative analysis of necrosis (p) (n = 5), Ly6G positive cells (q) (n = 5), and CD31⁺P-selectin⁺ cells (r) (n = 6) in liver tissue samples of (o). Data are presented as the mean ± SEM. P values are indicated as follows: * < 0.05; ** <0.01; *** <0.001; **** <0.0001, ns = no significance. P values were obtained using the unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.