Fig. 5: P-selectin inhibition reduces neutrophil infiltration and improves liver injury in BDL-induced cholestasis.

a Inhibitor administration to BDL mice via tail vein. b Gross morphology of livers from BDL mice treated as indicated (n = 5). c H&E showing necrosis in livers from (b), with red dotted box indicating the necrotic area. (d) Quantification of necrotic areas (n = 5). e Serum enzyme levels in mice from (b) (n = 5). f qPCR analysis of Selp and Sele in livers from (b) (n = 5). g IF staining of CD31 (green) and P-selectin (red) and quantification of CD31⁺P-selectin⁺ ECs in BDL livers from (b) (n = 5). h qPCR analysis of Ly6g and Ngp in livers from (b) (n = 5). i IHC of Ly6G and quantification in liver tissue samples from (b) (n = 5). j qPCR analysis of indicated genes in livers from (b) (n = 5). Data are presented as the mean ± SEM. P values are indicated as follows: * <0.05; ** <0.01; *** <0.001; **** <0.0001, ns = no significance. P values were obtained using the unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.