Fig. 7: Clinical relevance of the endothelial MYC/P-selectin regulation axis in patients with obstructive cholestasis (OC). | Nature Communications

Fig. 7: Clinical relevance of the endothelial MYC/P-selectin regulation axis in patients with obstructive cholestasis (OC).

From: Chenodeoxycholic acid modulates cholestatic niche through FXR/Myc/P-selectin axis in liver endothelial cells

Fig. 7

a Single-cell analysis workflow. b UMAP showing 9 major cell types. c Contour plot displaying changes in cell abundance. d Volcano plot showing significantly changed genes. e Enriched biological processes in OC ECs compared to controls. f UMAP showing the levels of MYC and SELP in ECs. g H&E and IHC for indicated proteins in liver samples, with arrows indicating Myc-positive ECs. Quantitative analysis of IHC for MYC, SELP, and MPO (n = 9, 16) (h), and Spearman correlation analysis (i). j Schematic diagram illustrating the molecular mechanism underlying the role of ECs in shaping the local immune microenvironment during obstructive cholestasis. Data are presented as the mean ± SEM. P values are indicated as follows: ** <0.01; *** <0.001; **** <0.0001. P values were obtained using the unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.

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