Fig. 5: Gene expression in erythroleukemic K562 cells. | Nature Communications

Fig. 5: Gene expression in erythroleukemic K562 cells.

From: FLT1 and other candidate fetal haemoglobin modifying loci in sickle cell disease in African ancestries

Fig. 5

a K562 cells were exposed to 1% oxygen over 10 days in culture. RT-qPCR was used to evaluate gene expression at predetermined time points. FLT1 (VEGFR1) and KDR (VEGFR2) gene expression are depicted, normalized to expression during culture in normoxic conditions. b RT-qPCR measurements of ALAS2 and CA9 expression, known markers induced by hypoxia. c RT-qPCR measurements of adult beta globin HBB, fetal haemoglobin genes HBG1/2, and a transcription factor involved in fetal haemoglobin regulation, KLF. RT-qPCR samples n = 3. Mean values of technical triplicates are presented. Error bars denote the standard error as calculated by Applied Biosystems qPCR software RQmax and RQmin. RQ relative quantitation of transcript levels.

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