Fig. 3: Overview of the C. beijerinckii pfl ZTP riboswitch consensus variant library.

a Sequence of the C. beijerinckii pfl ZTP riboswitch consensus variant library. b Correlation of replicate TECdisplay fraction terminator readthrough measurements for the 0 and 1 mM ZMP conditions. c Plots showing fraction terminator readthrough for the sequence variants shown in panel a as measured by TECdisplay with variable ZMP concentration. The ZMP concentration that was used for each data set is indicated above each plot. Variants are plotted along the x-axis by the difference in fraction terminator readthrough measured for the 1 mM ZMP and 0 mM ZMP conditions so that each variant is located at the same x-axis position in all plots. d–f Plots showing fraction terminator readthrough for the 0 mM ZMP (black) and 1 mM ZMP (red) conditions for all variants (d), variants in which P1, the pseudoknot, and the terminator are fully paired and can contain Watson–Crick or GU pairs (e), and variants in which P1, the pseudoknot, and the terminator are fully paired but the pseudoknot and terminator contain only Watson–Crick base pairs (f). g–j Dose-response curves for wt-like variants with strong pseudoknot and terminator pairs (g), pseudoknot mismatches and strong terminator pairs (h), strong pseudoknot pairs and terminator mismatches (i), and pseudoknot and terminator mismatches (j). TECdisplay data for the 0 and 1 mM ZMP conditions are from n = 2 replicates that were combined. TECdisplay data for the 31.6 μM, 100 μM, 316 μM, and 3.16 mM conditions are n = 1. Source data are provided as a Source Data file. PK pseudoknot, term terminator, WC Watson–Crick, MM mismatch.