Fig. 4: TECdisplay analysis of a 1,048,576-variant library.
From: Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display
a Sequence of the C. beijerinckii pfl ZTP riboswitch 1,048,576-variant library. b Histograms of the difference in fraction terminator readthrough observed in replicate experiments (replicate 1–replicate 2) for the 0 and 1 mM ZMP conditions. c Plots of the difference in fraction terminator readthrough observed in replicate experiments (replicate 1–replicate 2) against the combined sequencing depth of the replicates. d Correlation of fraction terminator readthrough measurements for a 32,768-variant subset of the 1,048,576-variant library with measurements made for the same variants as a standalone 32,768-variant library. e Correlation of fraction terminator readthrough measurements for the wild type, flip, strong, and weak pseudoknot variants made using the 1,048,576-variant and 32,768-variant libraries. All TECdisplay data are from n = 2 replicates. In panels d, e, replicate data were combined. Source data are provided as a Source Data file.
