Fig. 6: Overview of the alternative pseudoknot perturbation library.
From: Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display
a Sequence of the C. beijerinckii pfl ZTP riboswitch alternative pseudoknot perturbation library. b Plot showing fraction terminator readthrough in the absence of ZMP for variants with intact or disrupted terminator base pairs before and after variants that are prone to misfolding have been filtered. c, d Plots showing fraction terminator readthrough for variants that contain each of the sixteen intact terminator hairpins that occur in the library shown in panel a before (c) and after (d) variants that are prone to misfolding have been filtered. In panel d, C24 variants are separated from A24 and G24 variants because they exhibit context-dependent termination defects. TECdisplay data are from n = 2 replicates that were combined. Source data are provided as a Source Data file.
