Fig. 2: CAST systems are targetable in E. coli across genomic ORFs and intergenic regions.
From: Integration of therapeutic cargo into the human genome with programmable type V-K CAST
A Schematic representation of E. coli integration experiments. Plasmids harboring protein-coding elements (pHelper), integration cargo (pDonor), and sgRNA targeting locus (pGuide) are transformed into an engineered E. coli strain BL21(DE3). Antibiotic resistance is non-selective for integration. B On-target efficiencies at engineered loci targeting intergenic or coding targets (n = 3 biological replicates with one standard deviation from the mean). C On-target integration efficiencies at the engineered locus when multiplexed with integrations targeted to endogenous intergenic sites and protein-coding region targets (n = 3 biological replicates with one standard deviation from the mean). Source data are provided as a Source Data file.
