Fig. 5: ImmTAC redirection and IL-2 pre-treated T cells induce pro-tumoral macrophage reprogramming towards anti-tumoral phenotype. | Nature Communications

Fig. 5: ImmTAC redirection and IL-2 pre-treated T cells induce pro-tumoral macrophage reprogramming towards anti-tumoral phenotype.

From: Tebentafusp, a T cell engager, promotes macrophage reprogramming and in combination with IL-2 overcomes macrophage immunosuppression in cancer

Fig. 5

a T cell activation marker (CD69) and M2 macrophage marker (CD163) expression by T cells and macrophages respectively using untreated or IL-2 pre-treated T cells as effectors in ImmTAC re-direction co-culture assays with different effector T cell to M2 macrophage ratios (E:M2) (n = 4 biological replicates, Tukey’s multiple comparisons test, two-sided p values < 0.05 are shown). b, c Bulk RNA-seq analysis of sorted M2 macrophages from co-cultures. Ridge density plot of PC1 scores from PCA analysis of gene expression data (21.3% variance). M1 and M2 macrophages used as reference (n = 4 biological replicates) (b), heatmap gene expression plot using M1 and M2 associated genes. Top genes plotted based on variance of expression (n = 4 biological replicates) (c). d IFN-γ induced gene expression and pro-inflammatory cytokine expression levels of M2 cells sorted from ImmTAC-redirected co-cultures using untreated or IL-2 pre-treated effector T cells (M1 and M2 cells used as reference, n = 4 biological replicates). Abbreviations: E = Effectors, T = Targets, M1 = M1 macrophages, M2 = M2 macrophages, (UT) = Untreated T cells, (IL-2) = IL-2 pre-treated T cells, MFI = Mean Fluorescence Intensity.

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