Fig. 2: Composition of hepatic myeloid subsets in MASH mice and the change of the c-kit+ cDC1. | Nature Communications

Fig. 2: Composition of hepatic myeloid subsets in MASH mice and the change of the c-kit+ cDC1.

From: Microbiota-derived H2S induces c-kit+ cDC1 autophagic cell death and liver inflammation in metabolic dysfunction-associated steatohepatitis

Fig. 2

a Heatmap showing the median expression values and hierarchical clustering of the identified subpopulations. n = 5/group. b Composition of the myeloid cells. Color as shown in A. n = 5/group. c, d Gating strategy and representative flow cytometry dot plots of c-kit+CD103+ and c-kit+CD103- cDC1 in the liver. Lin includes CD3, CD19, NK1.1. n = 5/group. e Representative flow cytometry dot plots of hepatic c-kit+CD103+ cDC1 and c-kit+CD103- cDC1, as well as their proportion in XCR1+cDC1. n = 5/group. f Liver mIHC images stained with CD103 (red), c-kit (green), and XCR1 (white). Cell nuclei were stained with DAPI (blue). Magnification ×40, scale bar = 100 µm. Arrows indicate c-kit+CD103+ cDC1, and arrowheads indicate c-kit+CD103- cDC1. n = 5/group and two sections were stained from each liver sample. Data were represented as mean  ±  SEM and analyzed using a two-tailed unpaired Student’s t-test (b, d). Significance levels were reported as *P < 0.05, **P < 0.01. Source data were provided as a Source Data file.

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