Fig. 8: Gut-derived H₂S promotes the ACD of hepatic c-kit⁺ cDC1 and the development of MASH in mice.

a Top 10 KEGG enrichment of hepatic c-kit⁺ cDC1 from WT mice fed with NCD and WD for 30 W. n = 3/group. b GSEA analysis of Autophagy signaling. c Flow cytometry histograms of CYTO-ID (autophagic flux). n = 5/group. d Schematic of the H₂S delivery. e Autophagic flux analysis of hepatic XCR1⁺ cDC1. n = 5/group. f Correlation analysis between the concentration of H₂S and the autophagic flux of XCR1⁺ cDC1 in the liver. Spearman’s rank correlation coefficient test was performed. 95% confidence interval (0.7132 to 0.9658), P < 0.0001. g TEM showing the autophagosome and autolysosome in c-kit⁺ DC induced in vitro. n = 3/group. h Relative expression of p62 and LC3. n = 3/group. i MTT data showing the cell survival rate of c-kit⁺ DC induced in vitro. j MTT data showing the cell survival rate of c-kit⁺ DC in Atg5^flox/flox and Atg5^-/- mice. n ⁼ 5/group. Data were represented as mean ± SEM. P values calculated by a two-tailed unpaired Student’s t-test (g, j), one-way ANOVA with Tukey’s post hoc test (c, e, i) or two-way ANOVA with Tukey’s post hoc test (h). The screening criteria for KEGG were adjusted p < 0.05 and FDR value (q value) < 0.25, and the p value correction method was Benjamini-Hochberg (a). Significance levels were reported as *P < 0.05, **P < 0.01. a–c, groups sharing no common letters indicate statistically significant differences (P < 0.05), groups sharing partial letters indicate no significant difference (P > 0.05). 3MA 3-Methyladenine, FDR False Discovery Rate, MTT methylthiazolyl diphenyl-tetrazolium bromide. Source data were provided as a Source Data file.