Fig. 4: Loc-displacement for signal erasing and cyclic imaging of multiple targets.
From: POSA for fast, amplified and multiplexed protein imaging
a Schematic of fluorescence erasing step via Loc-displacement method. b The fluorescence images of Lamin A/C in HeLa cells were stained using OCPNs, then erased using the Loc-displacement reaction, and finally re-stained. The erasing process was completed within 3 minutes. c Cyclic imaging of Lamin A/C, mitochondria, α-tubulin, EGFR, GM130, and Vimentin by PFBT OCPNs during six cycles with the Loc-displacement reaction. d The merged image was reconstructed by false-colored fluorescent images from (c). e The zoom-in image of the marked region from (d). Source data are provided as a Source Data file.
