Fig. 5: NP-displacement for signal erasing and the reproducibility of repeated imaging of the same target.
From: POSA for fast, amplified and multiplexed protein imaging
a Schematic of fluorescence erasing step via the NP-displacement method. b The fluorescence images of Lamin A/C in HeLa cells were stained using OCPNs, then erased using the NP-displacement reaction, and finally re-stained. The erasing step was completed in around 30 minutes. c Fluorescent images of Lamin A/C in HeLa cells for six staining and erasing cycles via NP-displacement method. The fluorescence intensity of ten cells was determined by ImageJ software for each cycle. d Statistical results of relative fluorescence intensity from (c). The means ± standard deviations (error bars) of relative fluorescence intensity are presented based on ten marked cells during six cycles (n = 6). e The zoom-in images of the marked cells from (c) in different cycles. The fluorescent intensity value of each cell was determined by ImageJ software and marked at the top-left corner of each zoom-in image. Source data are provided as a Source Data file.
