Fig. 6: Single-cell multiplexing and amplified protein imaging by POSA.
From: POSA for fast, amplified and multiplexed protein imaging
a Fluorescent images of the nine proteins during three cycles. 1: anti-EGFR PA8-BS8 (PF-g-IS8 OCPNs), anti-Lamin A/C PA5-BS5 (PFBT-g-IS5 OCPNs) and anti-GM130 PA6-BS6 (PFTBT-g-IS6 OCPNs); 2: anti-mitochondria PA3-BS3 (PF-g-IS3 OCPNs), anti-α-tubulin PA1-BS1 (PFBT-g-IS1 OCPNs) and anti-Ki67 PA2-BS2 (PFTBT-g-IS2 OCPNs); 3: anti-TOMM20 PA4-BS4 (PF-g-IS5 OCPNs), anti-Lamin B1 PA9-BS9 (PFBT-g-IS9 OCPNs), and anti-Vimentin PA7-BS7 (PFTBT-g-IS7 OCPNs). The erasing process was conducted via the Loc-displacement method. b A merged false-color image from (a). c The zoom-in image of the marked region of (b). Source data are provided as a Source Data file.
