Fig. 1: Genetic background of RP and PE system design for the Pde6b mutation locus.
From: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa
a Genetic background of RP. b Schematic representation of the Pde6b nonsense mutation in rd1 mice. The C-to-A mutation in exon 7 of the Pde6b locus induces a stop codon and inhibits normal expression of the PDE6B protein. Prime editing was designed to correct the A base to the C base. c Flowchart of pegRNA and nsgRNA design. The sequence of exon 7 of the mouse Pde6b gene was input into three websites (PE-Designer, pegIT and PrimeDesign). Among the output results, four pegRNA and nsgRNA pairs were randomly selected from each website. The corresponding pairs were named pn1-12. The sequence of each pair was illustrated below.
