Fig. 5: RNA-seq analysis of genes relevant to phototransduction after PE correction.
From: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa
a KEGG enrichment analysis: downregulated phototransduction pathway (rd1 versus the WT group). Benjamini Hochberg were used. FDR < 0.05. b KEGG enrichment analysis: upregulated phototransduction pathway (the PE-edited group versus the rd1 group). Benjamini Hochberg were used. FDR < 0.05. c Volcano plot of genes differentially expressed between rd1 and WT mice. The red dots are upregulated genes, whereas the blue dots are downregulated genes. (t tests were used; |log2(fold change)|> 1 and p < 0.05). Genes relevant to the phototransduction pathway are highlighted. d Volcano plot of differentially expressed genes between PE-edited and rd1 mice. (t tests were used; |log2(fold change)|> 1 and p < 0.05). e The mRNA expression levels of genes relevant to the phototransduction pathway were detected in RNA-seq samples by RT‒qPCR. Representative data are presented as the mean ± SEM. Generally, n = 6 replicates (otherwise n = 4). Two-tailed unpaired t tests were used. ns, not significant; *p < 0.05; **p < 0.01.
