Fig. 6: A comprehensive characterization of EVcans proteins and pairwise comparative analysis between urinary EVs and tissue N-glycome.
From: Urinary extracellular vesicle N-glycomics identifies diagnostic glycosignatures for bladder cancer
a Pie charts show distribution of EVcans sites and EVcans proteins. b The co-occurrence probability of EVcans on the same protein or the same glycosylation site. c The heatmap demonstrates the differential expression of various glycoforms across the four groups. d Most dysregulated EVcans glycoforms were up-regulated in BC groups. e Hematoxylin and eosin (H&E) staining of the formalin-fixed, paraffin-embedded (FFPE) section used for MALDI-MSI. Scale bar: 2.5 mm. f Spatial clustering of N-glycans in formalin-fixed, paraffin-embedded (FFPE) sections based on MALDI-MSI outcomes. g The intensity distribution of differentially expressed N-glycans in tissues and EVs (Benjamini-Hochberg-adjusted ANOVA p < 0.05, log2 | FC | > 1). H&E staining data shown are from a single experimental replicate. Source data are provided as a Source Data file.
