Fig. 3: Renal epithelial cell-specific expression of YAPS127A induces pRCC in vivo.
From: Targeting the disrupted Hippo signaling to prevent neoplastic renal epithelial cell immune evasion
A Development of neoplasia and cancer in Pax8-YAPS127A transgenic mice. Left panel: Kidney size and morphology of mice with a Pax8 promoter-driven expression of YAPS127A (Pax8-YAPS127A) after inducing with doxycycline for ten days and five months. Age-matched wild-type mice and non-induced transgenic mice, which have similar phenotypes, were used as controls (CTL). Middle and right panels: Longitudinal (middle) and horizontal (right) section images (H-E stained) showing the size of kidneys from CTL and Pax8-YAPS127A mice after inducing gene expression with doxycycline for ten days and five months. Scale bar =1 mm. B Violin plots (with minima, maxima, median, and percentile information) showing changes in kidney weight, body weight, and kidney/body ratio in control (CTL) and Dox-induced (for 10 days or 5 months) Pax8-YAPS127A mice (n = 5 biological replicates for the groups of 10-days study; n = 7~9 biological replicates for the groups of 5-months study). Data were analyzed with unpaired t-tests (two-tailed). The P value of each comparison is presented on the top of the corresponding plot. C Representative H-E staining images from showing the histological morphology of kidneys from control (CTL) and Pax8-YAPS127A mice induced with doxycycline for 10 days and 5 months. The experiments with the similar observations have been independently repeated for more than ten times. Scale bar: 50 μm. D Immunohistochemical images showing expression of YAP1, Ki67, and vimentin in kidney tissues from control (CTL) and Pax8-YAPS127A mice induced with doxycycline for 10 days and 5 months. E H–E staining showing papillary structures derived from the acute Dox-induced neoplastic tissue (E1) and chronic intermittent Dox-induced cancerous tissues (E2–E4). Pax8-rtTA;Tet-on-YAPS127A mice were induced with doxycycline for two weeks (acute) or five months (chronic) before histological analyzes. Red arrows point to papillae observed in several Dox-induced transgenic mice. The experiments with the similar results have been independently repeated for more than ten times. Scale bar: 50 μm. Scale bar: 50 µm. Source data for (B) are provided in the Source Data file.
