Fig. 4: Single-cell RNAseq analyzes showing dynamic alteration of cellular landscape in renal tissue before and after pRCC development.
From: Targeting the disrupted Hippo signaling to prevent neoplastic renal epithelial cell immune evasion
A UMAP plot showing the overview of 18 major cell types identified in renal tissues in control (CTL, non-induced mice) and Pax8-rtTA;Tet-on-YAPS127A mice induced with doxycycline for seven days (D7) and four months (M4). B Violin plots of representative marker genes across the identified cell clusters. C UMAP plots showing the cellular components of renal tissues in Pax8-rtTA;Tet-on-YAPS127A mice induced with doxycycline for seven days (D7) and four months (M4). D Dynamic alteration of major cell components in renal tissues in control and doxycycline-induced Pax8-rtTA;tet-on-YAPS127A mice during hyperactivated YAP1-induced tumorigenesis. *: significantly different when compared to CTR. Statistical difference were performed with scCODA with FDR = 0.05. Please note that mice in the M4 group were generated using a low dose/chronic induction protocol in order to avoid acute neoplasia-associated kidney failure.
