Fig. 7: MDSC depletion blocks the development of pRCC early lesions.
From: Targeting the disrupted Hippo signaling to prevent neoplastic renal epithelial cell immune evasion
A FACS showing the effect of clodronate (CLD) on the accumulation of MDSCs in the renal tissues of doxycycline-induced Pax8-rtTA;tet-YAPS127A mice. Mice were treated with Dox ± CLD for 2 weeks before FACS analysis. Please note that the administration of CLD significantly reduced MDSCs in the neoplastic renal tissues. The violin plot (with minima, maxima, center, and percentile, etc.) on the right shows changes in MDSC ratios (n = 4-5 biological replicates). Data were analyzed with One-Way ANOVA followed by the Tukey multiple comparisons test. PBS + H2O vs. PBS + DOX: P < 0.0001 (****); PBS + DOX vs. CLD + DOX: P = 0.0081 (**). B Morphology and histology of kidneys in Pax8-rtTA;tet-YAPS127A mice treated with or without doxycycline (Dox) and clodronate (CLD) for 2 weeks. Scale bar: 25 µm. C Violin plots (with minima, maxima, median, and percentile, etc.) showing the effect of CLD on the body, kidney, and spleen weights of the Pax8-rtTA;tet-YAPS127A mice treated with or without Dox for 2 weeks (n = 5 biological replicates). Data were analyzed with One-Way ANOVA followed by the Tukey multiple comparisons test. Statistics for the kidney weight: PBS + H2O vs. PBS + DOX: P < 0.0001 (****); PBS + DOX vs. CLD + DOX: P = 0.0001 (***). Statistics for the kidney somatic index: PBS + H2O vs. PBS + DOX: P < 0.0001 (****); PBS + DOX vs. CLD + DOX: P = 0.0148 (*).D) Representative images (from six biological repeats) showing the effect of CLD on the expression of CD45 (pan-immune cell marker) and F4/80 (macrophage marker) in renal tissues of the Pax8-rtTA;tet-YAPS127A mice treated with or without Dox for 2 weeks. Scale bar: 25 µm. Source data for (A and C) are provided in the Source Data file.
