Fig. 9: TEAD inhibitor MGH-CP1 blocked YAP-induced kidney neoplasia.
From: Targeting the disrupted Hippo signaling to prevent neoplastic renal epithelial cell immune evasion
A Structure of the MGH-CP1 molecule. B Kidney size and morphology of control mice (Ctrl), Pax8-YAPS127A mice (YAP, Dox-induced Pax8-rtTA;Tet-on-YAPS127A mice), and Pax8-YAPS127A mice treated with MGH-CP1 (YAP + MGH-CP1). Ruler scale: 1 mm. C Changes of body weight in control mice (Ctrl), Pax8-YAPS127A mice (YAP), and MGH-CP1 treated Pax8-YAPS127A mice (YAP + MGH-CP1). Data were represented as the mean ± SEM (Ctrl: n = 10; YAP: n = 12; YAP + MGH-CP1: n = 18 biological replicates). Data were analyzed with One-Way ANOVA followed by the Tukey multiple comparisons test. Statistics for the kidney weight: CTL vs. YAP: P < 0.0001 (****); YAP vs. YAP + MGH-CP1: P < 0.0001 (****). Statistics for the kidney/body ratio (%): CTL vs. YAP: P < 0.0001 (****); YAP vs. YAP + MGH-CP1: P = 0.0001 (***).D Representative H-E staining images showing changes in kidney size and histological morphology in control mice (Ctrl), Pax8-YAPS127A mice (YAP), and MGH-CP1 treated Pax8-YAPS127A mice (YAP + MGH-CP1). E Survival curves of control mice (Ctrl), Dox-induced Pax8-YAPS127A mice (YAP), and Pax8-YAPS127A mice treated with MGH-CP1 (YAP + MGH-CP1). F representative H-E staining images showing renal tissue histology of control mice (Ctrl), Pax8-YAPS127A mice (YAP), and MGH-CP1 treated Pax8-YAPS127A mice (YAP + MGH-CP1). The experiments with the similar results have been independently repeated for more than ten times. Scale bar: 50μm. G IHC images showing the expression of YAP1, CD45, and MDSC biomarkers (Arginase 1, F4/80, and VISTA) in renal tissues from control mice (Ctrl, n = 6), Pax8-YAPS127A mice (YAP, n = 6), and MGH-CP1 treated Pax8-YAPS127A mice (YAP + MGH-CP1, n = 6). Source data for panels C and E are provided in the Source Data file.
