Fig. 6: Composition of immune cells in the tumor microenvironment shifted by RDPNs@diABZIs.

a, b Representative flow cytometry histogram and relative quantification of CD86 expressions by DCs (CD45⁺CD11c⁺CD86⁺) in the TDLNs. n = 3 biologically independent animals per group. c, d Representative flow cytometric analysis and quantification of CD86 expressions in M1-like macrophages (CD45⁺CD11b⁺F4/80⁺CD86⁺) in tumors. n = 3 biologically independent animals per group. e, h Representative flow cytometry histogram and quantification of CD206 expressions in M2-like macrophages (CD45⁺CD11b⁺F4/80⁺CD206⁺) in tumors. n = 3 biologically independent animals per group. f, j, Representative flow cytometric analysis and relative quantification of CD8⁺ T cells (CD3⁺CD8⁺) in the TDLNs. n = 3 biologically independent animals per group. g, k Representative flow cytometric analysis and relative quantification of CD8⁺IFN-γ⁺ T cells (CD45⁺CD8⁺IFN-γ⁺) in the TDLNs. n = 3 biologically independent animals per group. i Quantification of NK cells (CD45⁺CD49b⁺) in spleen. n = 3 biologically independent animals per group. l Serum cytokine concentration of IFN-γ, TNF-α and Granzyme B. n = 3 biologically independent animals per group. Data were expressed as means ± SD. (G1: PBS, G2: NPNs, G3: RDPNs, G4: diABZIs, G5: RDPNs@diABZIs. DCs, Dendritic cells. NK cells, Natural killer cells. One-way ANOVA with Tukey’s post hoc test (b, c, h, i, j, k, l). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.