Fig. 1: Map-based cloning of Ne1.
From: Genomic structural variation in an alpha/beta hydrolase triggers hybrid necrosis in wheat
a Phenotypes of necrotic line M114 (Ne1Ne1Ne2Ne2), Zhoumai22 (ZM, ne1ne1Ne2Ne2), and F1 plant derived from M114 × ZM at the grain filling stage in the field. Scale bar in plant, 8 cm; Scale bar in leaf, 2 cm. b Fine genetic map of Ne1 constructed using the F2 populations derived from M114 × ZM. c Physical map of Ne1 region according to Chinese Spring (CS) reference genome sequence IWGSC RefSeq v2.1. The blue oval indicates Ne1. d Fine genetic map of Ne1 constructed using segregation populations derived from Zhengnong17 (ZN17, ne1ne1Ne2Ne2) and Yangbaimai (YBM, Ne1Ne1ne2ne2). Ne1 is highlighted in blue. e Gene structure and sequence variation of ABH.1 gene. Black boxes and gray lines indicate exons and introns, respectively. The variation of coding sequence (c) and amino acid (p) sequences between CS, YBM, and M114 are indicated. f Protein structure prediction of ABH.1. Hydrolase domain, AAA ATPase with vWA domain, and membrane protease YdiL domain are represented in orange, green, and purple, respectively. The signal peptide is indicated in red. Source data are provided as a Source Data file.
