Fig. 2: Functional validation of ABH.1 by mutagenesis.
From: Genomic structural variation in an alpha/beta hydrolase triggers hybrid necrosis in wheat
a, c Phenotypes of non-necrotic mutants generated from the necrotic NIL-Ne1 (Ne1Ne1Ne2Ne2) and M114 (Ne1Ne1Ne2Ne2) background by ethyl methanesulfonate (EMS) treatment. Gene structure and EMS mutant analysis of ABH.1 (b) and Ne2 (d). The positions of the EMS-derived loss-of-function mutations are indicated by black lines. Black boxes and gray straight lines represent exons and introns, respectively. The coding sequence (c) changes and their predicted effects on the amino acid (p) are indicated. Scale bar in (a and c), 1 cm. Mutation names in red indicate nonsense mutations. Source data are provided as a Source Data file.
