Fig. 3: Transgenic validation of ABH.1.
From: Genomic structural variation in an alpha/beta hydrolase triggers hybrid necrosis in wheat
a Structure of proUbi::ABH.1YBM used for transformation of wheat cultivar Fielder. The construct contains the ABH.1YBM coding sequence (CDS) and maize ubiquitin promoter region (proUbi). LB left border; RB, right border; Nos Ter, termination sequence of the Agrobacterium tumefaciens nopaline synthase gene. b Genetic complementation of ABH.1 and Ne2 was performed by crossing independent transformants (OEABH.1YBM#1-#4) carrying proUbi::ABH.1YBM and wheat cultivar ZZ6903 with the ne1ne1Ne2Ne2 genotype, which induced the expression of hybrid necrosis. Representative leaves of four F1 hybrids and their corresponding parental lines are presented. Scale bar, 1 cm. c Structure of proABH.1M114::ABH.1M114 used for transformation of the Ne2 overexpression transgenic line OE-T1−1-1 (ne1ne1Ne2Ne2, OENe2) in the Fielder genetic background and the F1 plants (ne1ne1Ne2ne2) of ZM × Fielder, respectively, by Agrobacterium-mediated transformation. The construct proABH.1M114::ABH.1M114 consisted of a 13,118 bp genomic fragment of ABHM114 from M114, comprising 8157 bp the entire gene body, 2406 bp upstream native promoter and 2,556 bp downstream regulatory sequences, respectively. d Phenotypes of OENe2, positive transgenic lines (OENe2 + COMABH.1M114#1-#3) in OENe2 background, ZM, positive transgenic lines (Ne2Ne2 + COMABH.1M114#1-#3, and ne2ne2 + COMABH.1M114#1) in ZM × Fielder background. Scale bar, 1 cm. Source data are provided as a Source Data file.
