Fig. 3: The prolonged-expression period of Zm00001d044416 was related to the leafy phenotype.

a, b Expression levels of Zm00001d044416 in different tissues (a), and in shoot tips during plant growth from V1 (one expanded leaf) to V7 (b) in NIL^Z-Lfy1 and NIL^Z58. Tassel collected at tassel emergence stage (VT); unpollinated ear and husk collected at silking stage (R1); DAP12, 12 days after pollination; G5, 5 days after germination. Each experiment was repeated three times independently. The error bars indicate mean ± SD, and a two-tailed Student’s t-test was used to determine P-values between the two NILs; c, d In situ hybridization of Zm00001d044416 in shoot tips of NIL^Z58 (c) and NIL^Z-Lfy1 (d) at V4 stages. SAM, shoot apical meristem; the red arrows indicate primary vascular bundles. Bar = 200 μm. Source data are provided as a Source Data file.