Fig. 4: Zm00001d044416 encodes an AAA+ ATPase that interacts with itself in mitochondria.
From: A LTR retrotransposon insertion leads to leafy phenotype in maize by elevating ZmOM66 expression
a The ZmOM66 protein contains an N-terminal domain and an AAA+ ATPase domain; b Phylogenetic relationships of ZmOM66 and its homologs in nematode (C. elegan), human (H. sapens), yeast (S. cerevisiae), Arabidopsis (At), rice (Os), and maize (Zm). ZmOM66 homologs were aligned using bootstrap consensus in MEGA 7.0 software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. Scale bar = average number of amino acid substitutions per site; c–e Subcellular localization of the ZmOM66 protein in tobacco leaf epidermal cells (c), maize leaf protoplast (d) and Arabidopsis root hairs (e); f Yeast two-hybrid assay of the interaction between two ZmOM66 proteins. The interactions between pGADT7-T and pGBKT7-53 and between pGADT7-T (AD) and pGBKT7-Lam (BD) were used as positive and negative controls, respectively. (g, h) Luciferase complementation imaging (LCI) (g) and bimolecular fluorescence complementation (BiFC) assay (h) of two ZmOM66 proteins in tobacco leaves. The fluorescence signals represent the interaction activities. Bar = 20 μm. In c–e and h, each experiment was repeated three times independently.
